Approach
- Marmoset (MAR) antigen (cytokine, hormone) design, plasmid development and antigen production.
- Immunization of mice and serology analysis.
- Hybridoma production and monoclonal antibody (mAb) screening.
- Small scale mAb production and purification.
- Identification of optimal mAb pairs for immunoassays and single mAbs for Flow Cytometry.
- Subcloning or sequencing, small-scale production and purification of optimal mAbs.
- Large scale mAb production, purification, conjugation, QC, benchmarking (if possible), titration, stability tests, ELISA standard development.
- Validation with MAR samples in relevant assays.
- Validation with owl monkey (OWL) and squirrel monkey (SQM) samples in relevant assays.
- Availability of validated mAbs for researchers.
听
听